What is the role of DNA ligase in the elongation of the lagging strand during DNA replication? It joins Okazaki fragments together. … An enzyme that unzips the double helix of DNA at the replication forks. What is the use of ligase? Ligase, an enzyme that uses ATP to formRead More →
The phosphate group is attached to the 5′ carbon of one nucleotide and the 3′ carbon of the next nucleotide. In its natural state, each DNA molecule is actually composed of two single strands held together along their length with hydrogen bonds between the bases. Where are the phosphates located?Read More →
Dissociation of the double stranded DNA helix into single coils is referred to as DNA melting. It can be accomplished by simply heating double stranded DNA. Therefore, in principle the more G and C in the sequence the higher temperature is needed to melt a given DNA fragment. … WhatRead More →
There are two kinds of DNAs, single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA). … While DSBs involve in binding to particular dsDNA sequences, to modulate the process of transcription, to cleave DNA molecules, or to be involved in chromosome packaging and transcription in the cell nucleus, etc. What is single-strandedRead More →
It is done by an enzyme called RNA Polymerase (RNA Pol). RNA Pol only reads one of the two DNA strands as it makes the RNA molecule. The double-stranded DNA molecule is split apart and RNA Pol binds to one strand, which it will read and copy. This strand isRead More →
In any of these electrophoresis techniques, the locations of the DNA or RNA fragments in the gel can be detected by various methods. One common method is adding ethidium bromide, a stain that inserts into the nucleic acids at non-specific locations and can be visualized when exposed to ultraviolet light.Read More →
Thus, acetylation of histones is known to increase the expression of genes through transcription activation. … This leads to decreased levels of gene expression and is known as gene silencing. Acetylated histones, the octomeric protein cores of nucleosomes, represent a type of epigenetic marker within chromatin. Is histone methylation inherited?Read More →
Our human evolutionary lineage includes many species and relatives, including Neanderthals and Denisovans. Scientists already knew that modern humans interbred with Neanderthals and Denisovans 40,000 to 60,000 years ago. Which modern humans have the most Neanderthal DNA? East Asians seem to have the most Neanderthal DNA in their genomes, followedRead More →
Adenoviruses are a group of viruses that typically cause respiratory illnesses, such as a common cold, conjunctivitis (an infection in the eye that is sometimes called pink eye), croup, bronchitis, or pneumonia. In children, adenoviruses usually cause infections in the respiratory tract and intestinal tract. Does adenovirus contain DNA? TheRead More →
Interphase is a phase of the cell cycle, defined only by the absence of cell division. During interphase, the cell obtains nutrients, and duplicates (copies) its chromatids (genetic material). During what phase is DNA replication? S phase is the period during which DNA replication occurs. In which phase is theRead More →