What Does Fetal Bovine Serum Do?

Fetal bovine serum (FBS) is a common component of animal cell culture media. It is harvested from bovine fetuses taken from pregnant cows during slaughter. FBS is commonly harvested by means of a cardiac puncture without any form of anaesthesia.

What does fetal bovine serum contain?

Fetal bovine serum (FBS) is the liquid fraction of clotted blood from fetal calves, depleted of cells, fibrin and clotting factors, but containing a large number of nutritional and macromolecular factors essential for cell growth.

Why is fetal bovine serum expensive?

Australian FBS prices are higher because Australia is perceived to be a “safer” origin for BSE and cattle viruses. Additionally, the OIE veterinary authorities have declared all blood and blood product, regardless of country of origin, to be safe in terms of BSE. …

Does cultured meat use fetal bovine serum?

FBS is the liquid fraction which remains after the blood drawn from a bovine fetus coagulates. Though it is rich in components conducive to cell growth, FBS cannot be used in the production of commercial cultured meat products.

How do you get fetal bovine serum?

Fetal bovine blood is collected from deceased pregnant cows in government approved facilities. Blood is drawn via cardiac puncture from the expired fetus in a closed, aseptic system using best practices to regulate hemoglobin and endotoxin levels. The blood is refrigerated to encourage clotting.

What is unborn calf blood used for?

The serum which is obtained from fetal blood, is used for scientific purposes such as vaccine production. RSPCA Australia advocates the use of non-animal alternatives instead of blood being collected from fetuses and for these to be developed as quickly as possible.

What is the difference between fetal bovine serum and fetal calf serum?

Fetal Bovine Serum is the same as Fetal Calf Serum. There is no difference and semantics have no place in science, and anyone working with cells should know that. … It comes from calves already born and typically doesn’t have the levels of growth factors that FBS contains.

Is there glucose in fetal bovine serum?

hi,all. the cells will be suffered high glucose DMEM (25.0 mM glucose) or glucose-free DMEM (no glucose) 24h, low glucose as control(5.55 mM glucose). However, the serum contain glucose.

When was fetal bovine serum first used?

In the late 1950s, Theodore Puck first introduced the use of FBS with the purpose of encouraging cellular proliferation. For several decades, the use of fetal bovine serum has been ubiquitous across laboratories worldwide.

How do you inactivate fetal bovine serum?

Technical Tips 1 – Heat Inactivation of Fetal Bovine Serum

  1. Thaw the serum slowly to 37°C and mix the contents of the bottle thoroughly.
  2. Place the thawed bottle of serum into a 56°C water bath containing enough water to immerse the bottle to just above the level of the serum.
  3. Begin timing for 30 minutes.

What is fetal clone II?

HyClone FetalClone II is an economical alternative to fetal bovine serum (FBS) delivering both cost-effectiveness and high performance. Commonly used in cell culture and bioprocessing applications, FetalClone II is a supplement to enrich cell culture performance.

How do I test my FBS lot?

METHOD

  1. Test each batch of FBS against your present lot. …
  2. For each lot of serum to test, prepare 30 mL of 10% FBS in medium (e.g., Dulbecco’s modified Eagle’s or RPMI 1640) without any further additives. …
  3. Wash the three test cell lines (one myeloma and two hybridomas) in medium without serum.

What are the reasons for including Foetal bovine serum FBS in mammalian cell cultures?

Benefits of fetal bovine serum

Serum is added to culture medium at a concentration of 2-10% to provide attachment factors, nutrients, and hormones for mammalian cells, as well as to be a buffer against disruptions like pH changes and endotoxins.

Does fetal bovine serum contain antibodies?

Fetal bovine serum is the most widely used serum-supplement for the in vitro cell culture of eukaryotic cells. This is due to it having a very low level of antibodies and containing more growth factors, allowing for versatility in many different cell culture applications.

Can I use horse serum instead of FBS?

Therefore horse serum is the ideal supplement in nutrient media used for in vitro growth of specific hematopoietic progenitors. Applications: Cost effective alternative to FBS. growth of specific hematopoietic progenitor stem cells.

What difference is there between Foetal and newborn serum?

There is no difference between FBS and FCS, it is the same serum with name preference. However, the ‘F’ for fetal is very important, and catalog numbers from different companies are critical for cell culture.

What is bovine origin?

Bovine comes from the Latin word for “cow”, though the biological family called the Bovidae actually includes not only cows and oxen but also goats, sheep, bison, and buffalo. So bovine is often used technically, when discussing “bovine diseases”, “bovine anatomy”, and so on.

Why do they slaughter pregnant cows?

Reasons for sending pregnant cows for slaughter include presumed infertility (cattle erroneously considered to be non-pregnant), low production, and mastitis .

Do they slaughter pregnant cows?

The slaughter of a small number of pregnant cows is an inherent, unavoidable part of meat and milk production. Once a cow is slaughtered, the fetus inevitably will die. The unnecessary suffering of fetuses in slaughterhouses is avoided when they are retrieved and properly handled by serum industry technicians.

What is cosmic calf serum?

HyClone Cosmic Calf serum is a high-performing bovine calf serum that has been fortified with iron and a proprietary blend of growth-promoting factors. The result is a naturally derived, high-quality engineered serum that has demonstrated excellent performance with a broad range of cell types.

Can FBS be replaced?

Serum-free media or human platelet lysates can replace FBS when culturing cells.

What is horse serum?

Horse Serum, or Equine Serum, is a nutrient-rich fluid derived from the blood of controlled donor horse herds, but does not contain red blood cells or other clotting components.