There are four steps involved in obtaining a good quality specimen for testing: (1) preparation of the patient, (2) collection of the specimen, (3) processing the specimen, and (4) storing and/or transporting the specimen.
When do you take a vomit sample?
Vomit samples should only be collected after consultation with the laboratory or PHU. (Collection and storage of vomit specimens are the same as those for faecal specimens). Specimens should be collected in an appropriate sterile laboratory container.
How do you collect microbiological samples?
Collect specimen under good lighting. Pass the swab firmly over or into an area of suspected infection and obtain a sample of exudate, drainage, or purulent discharge if these are present. Return the swab to the transport tube and break the media ampule at the base of the tube to moisten the swab.
What is sample collection in microbiology?
Specimen collection requires withdrawing blood, cerebrospinal fluid, collecting urine, or swabs from mucosal surfaces. Specimen collection is performed using aseptic techniques to ensure sterility of the sample and avoid contamination from bacteria or other bodily fluids.
What are the different methods for collection of blood sample?
Three popular methods of blood collection are:
- Arterial Sampling.
- Venipuncture Sampling.
- Fingerstick Sampling.
How do you collect a stool sample?
To collect the sample:
- label a clean, screw-top container with your name, date of birth and the date.
- place something in the toilet to catch the poo, such as a potty or an empty plastic food container, or spread clean newspaper or plastic wrap over the rim of the toilet.
What types of samples might be collected from patients to use for laboratory testing?
Most often, all that is required is a blood sample. However, samples of urine, saliva, sputum, feces, semen, and other bodily fluids and tissues also can be tested. For some samples, they can be obtained as the body naturally eliminates them.
How do you collect samples?
Many samples are collected by running a sterile swab over an affected area. Throat, nasal, vaginal, and superficial wound cultures are obtained in this way. Often a trained health professional will take the swab or sometimes you may be asked to do it yourself.
How are the specimen collected and handled?
Hands should be washed before and after specimen collection. In line with standard precautions, appropriate personal protective equipment should be worn when collecting or handling specimens. Specimens should be collected in sterile containers with close fitting lids to avoid contamination and spillage.
What is specimen collection?
Specimen collection is the process of obtaining tissue or fluids for laboratory analysis or near-patient testing.
How do you collect a throat swab sample?
Rub the swab on the back of the throat, on the tonsils, and in any other area where there is redness, inflammation or pus. n Touch the swab tip to the tonsil area. n Rub the swab tip quickly and firmly over this area to obtain a good sample. n Remove swab from mouth (without touching any surface).
How do you collect a midstream urine specimen?
To collect the urine sample: Keeping your labia spread open, urinate a small amount into the toilet bowl, then stop the flow of urine. Hold the urine cup a few inches (or a few centimeters) from the urethra and urinate until the cup is about half full. You may finish urinating into the toilet bowl.
What type of urine specimen is collected for urinalysis?
Specimen collection: 1. Routine or random sample: The patient is given a non-sterile collection container and instructed to collect a midstream specimen in the container. This type of specimen is routinely used for urinalysis and may not be used for a culture and sensitivity.
How do you collect a stool sample for ova and parasites?
The stool should be collected into a clean, dry plastic jar with a screw-cap lid. For best results, the stool should be brought to the lab right away. If this isn’t possible, the stool should be stored in preservative provided by the lab and then taken there as soon as possible.
What must be avoided when collecting a stool specimen?
If possible, avoid the following within 48 hours before stool collection: Pepto Bismol, Maalox, mineral oil, antacids (Rolaids, Tums), Kaopectate.
How fresh Should a stool sample be?
Storing a stool sample
If you can’t hand the stool sample in immediately, you should store it in a fridge, but for no longer than 24 hours. Place the container in a sealed plastic bag first. Stool samples must be fresh – if they aren’t, the bacteria in them can multiply.
How do you collect capillary blood samples?
Capillary blood is obtained by pricking a finger in adults and a heel in infants and small children. The specimen is then collected with a pipette, placed on a glass slide or a piece of filter paper, or is absorbed by the tip of a microsampling device.
How do you collect blood evidence?
Place thread on bloodstain with a pair of clean forceps or a clean cotton swab. Roll the thread on the bloodstain, so the stain is absorbed onto the thread. Repeat until a minimum of four threads are collected. Place the threads (and swabs, if used) in a secure area and allow them to air dry.
What are the 3 main veins to draw blood?
The most site for venipuncture is the antecubital fossa located in the anterior elbow at the fold. This area houses three veins: the cephalic, median cubital, and basilic veins (Figure 1).
How do you collect an environmental sample?
Collect a sample by vigorously rubbing the swab with firm pressure approximately 10 times in a vertical, up and down motion following a “Z” pattern. Turn swab to opposite side and swab approximately 10 times in a horizontal, side to side motion over the surface area being sampled.
What is environmental sampling?
Collecting samples involves the isolation and careful gathering of specimens of interest in a given environmental study. Important environmental parameters such as pH, dissolved oxygen, electrical conductivity, and hydrological conditions aid in classifying the collection environment.
Can a dry swab pick up bacteria?
A comparative study of the wet and dry swab in its ability to detect the organism was performed against critically ill patients, and it was found that there were no statistically significant differences between the two different methods. The wet swab did not show increased sensitivity compared to dry one.