Q: What CBC parameters are affected when the specimen is lipemic? A: Lipemia in a blood specimen used for clinical evaluation can cause significant interference with obtaining accurate test values. Lipemia creates turbidity of a sample and is a result of the accumulation of lipid particles.
What is affected by lipemia?
Lipemia interferes with hematology tests by the following mechanism by light scattering. This affects the following results: Hemoglobin and hemoglobin-related indices: Results in falsely increased absorbance readings of hemoglobin, causing a falsely high measurement.
How does lipemia sample affect lab results?
How Does Lipemia Impact Laboratory Testing? Lipemia results from sample turbidity from accumulation of lipoprotein particles and can interfere with laboratory analysis by several mechanisms. First, lipemia can increase absorption of light and thereby decrease light transmittance used for spectrophotometric analysis.
Why does lipemia in specimen cause an inaccurate hemoglobin value?
Lipemic specimens contain high levels of triglycerides consisting of chylomicrons and very low-density lipoprotein particles, which in turn cause turbidity. This turbidity interferes with light scatter and the absorption of light, resulting in a false increase of hemoglobin determinations.
How does lipemia affect creatinine?
Clinically significant interferences were found for phosphorus, creatinine, total protein and calcium. Conclusion: Lipemia causes clinically significant interferences for phosphorus, creatinine, total protein and calcium measurement and those interferences could be effectively removed by ultracentrifugation.
Is glucose affected by lipemia?
In this range of lipemia, calcium, chloride, glucose, iron, magnesium, phosphorus, total protein, TIBC, and urea had a significant bias in results ( value < 0.05 for calcium, phosphorus, and magnesium). In this group, using serum blank was helpful for glucose and chloride measurement, unlike serum dilution.
What causes lipemic blood?
The most common cause of lipemia is nonfasting, with recent ingestion of lipid-containing meal. More severe lipemia results from a disease condition causing hypertriglyceridemia (eg, diabetes, genetic hyperlipidemia) or recent intravenous infusion of a lipid emulsion.
What does it mean if your blood is lipemic?
Lipemia is presence of a high concentration of lipids (or fats) in the blood. When donated blood is lipemic it causes the plasma-containing products to have a milky appearance.
How does lipemia affect electrolytes?
Considering 0-350 mg% of triglyceride as the reference, electrolytes concentration mostly decreased over increasing lipemia. Beyond triglyceride concentration of 650mg%, this decline in electrolytes concentration was statistically significantly for samples in all subgroups.
What on the CBC will falsely elevate the Wbcs?
Falsely elevated WBC values may be due to aggregated platelet clumps or incomplete rbc lysis. Instruments are typically able to flag these, though they may not be able to correct for them automatically. In the case of aggregated platelets, the clumping increases the size of particles to be comparable to wbcs.
How does serum remove lipemia?
Conclusions: High-speed centrifugation (10,000×g for 15 minutes) can be used instead of ultracentrifugation to remove lipemia in serum/plasma samples.
Does lipemia affect triglycerides?
Some laboratories include triglyceride concentration measurement in a rough assessment of degree of lipemia. … Therefore, an increased amount of glycerol in the sample will result with falsely increased concentration of triglycerides.
Why is lipemic sample not used in serologic testing?
The possible mechanism of interference by hemolysis or lipemia in serologic assays may be as follows: Interference with antigen-antibody reaction. … Very severe lipemia may make it difficult for accurate volume measurement in assays requiring precise sample measurement. Interference with color measurement in assays.
What causes falsely elevated hemoglobin?
Medical conditions that can cause high hemoglobin levels include: Polycythemia vera (the bone marrow produces too many red blood cells) Lung diseases such as COPD, emphysema or pulmonary fibrosis (lung tissue becomes scarred) Heart disease, especially congenital heart disease (the baby is born with it)
What is meant by lipemic?
Lipaemia is defined as an abnormally high concentration of lipids in the blood, usually in the form of very low density lipoproteins (VLDLs) or chylomicrons. Characteristically the blood plasma may appear white or milky in colour due to the presence of fat.
What is a lipemic blood sample?
What Are Lipemic Samples. Lipemic samples are patient specimens that have a higher fat content in them, so their blood is a little more milky, thicker. Whereas, usually when you spin down whole blood, it will look like this. It has yellow, clear serum or plasma on top of the red cells.
Does lipemia affect ferritin?
Results are not known to be affected by icteric, hemolyzed or lipemic specimens. Ferritin reference ranges are assay-specific. The Roche Diagnostics kit specifies expected values of 30-400 ng/mL for men and 15-150 ng/mL for women.
What is a HIL blood test?
In conclusion, automated assessment of hemolysis, icterus, & lipemia (HIL) provides the laboratory a standardized, reproducible and efficient tool to detect possible interference related to sample integrity.
How does hemolysis affect bilirubin?
Summary. In the van den Bergh reaction, hemolysis in serum samples results in decreased azobilirubin color development and hence in measured bilirubin levels which are falsely low.
Does menstruation affect creatinine levels?
Conclusion: Despite the statistically significant luteal phase increase, creatinine clearance does not change in a clinically important manner during the normal menstrual cycle. Specifically, there is no substantial decrease in creatinine clearance around the time of ovulation.
Can fenofibrate increased creatinine levels?
Fenofibrate therapy is commonly prescribed for the clinical management of elevated triglyceride levels in diabetic patients, but has been noted to cause an elevation in serum creatinine concentrations.
Why is Cyanmethemoglobin method preferred by most laboratories for hemoglobin determination?
The haemoglobin is estimated with the help of cyanmethemoglobin curve. The advantages of this method are i) error due to subjective visual matching is avoided as spectrophotometer is used and hence reading is precise and reliable, ii) measures all forms of haemoglobin except sulphaemoglobin.
Is the destruction of red blood cells with the release of hemoglobin into the plasma?
Hemolysis is the destruction of red blood cells. Hemolysis can occur due to different causes and leads to the release of hemoglobin into the bloodstream.